P-393 The mitochondrial targeted antioxidant Mitoquinol increases survival of isolated human preantral follicles in vitro

Abstract Study question Does Mitoquinol have a protective effect on survival and mitochondrial oxidative stress in cultured human preantral follicles? Summary answer significantly increased of follicles for 8 days decreased expression genes related to the response. What is known already Culturing ovarian promising new source mature oocytes fertility preservation, as well key model system investigate fundamental biology. Nevertheless, only handful studies yielded from stage optimizing culture systems challenging due scarcity material. an antioxidant that contains form Coenzyme Q10 called ubiquinone has been modified selectively target protect mitochondria damage. In oocytes, presence IVM media nuclear maturation protected against chromosomal misalignment. design, size, duration Human (n = 213; mean diameter: 71 µm; range: 26-189 µm) were isolated medulla tissue. Preantral mechanically enzymatically isolated, encapsulated 0.5% alginate three experimental groups: Control 68); 50nM 71), 250nM 74). The primary endpoints follicular growth survival. Secondary included gene hormone analysis spent media. Participants/materials, setting, methods Ovarian tissue was donated by 5 patients (aged 30-37 years) undergoing unilateral oophorectomy cryopreservation. Follicular assessed every second day during microscopy. For bigger than 95 µm, AMH Estradiol concentrations measured ELISA 8. Finally surviving snap-frozen analysed qPCR; genes: HSP-60, TFAM apoptosis BAX/BCL-2. Main results role chance After culture, rate group (68%; n 51/74) higher control (49%; 32/68; p 0.0344) (54%; 39/71; 0.0443). more pronounced subset small (Day0 < 70 135) which (65%; 31/47) (41%; 18/43; 0.005) (44%; 20/4; 0.019). average similar groups (Control: 40.7 ± 17.4 50nM: 36.9 19 μm; 250nM: 36.4 ±16.5 μm). A significant positive association between diameter follicle concentration observed (AMH: 0.0215, R 0.43 Estradiol: 0.0413, 0.38) but no differences found groups. relative involved response lower compared 250mM (HSP-60 0.018; 0.013) 0.056; 0.071). Moreover, ratio BAX/BCL2 Limitations, reasons caution larger sample size required confirm statistical extrapolate primordial, primary, secondary follicles. Furthermore, long-term are determine impact antral development oocyte Wider implications findings Our show can be used since it reduced Trial registration number Not appliclable